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Category |
Plasmid Name |
Description |
Map (pdf) and sequence (text file, abbreviated gbk format): will open in new window |
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Basic transformation (limited cloning sites) |
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Not all plasmids described in our publications are listed here. Please let us know if you are interested in a plasmid that is not shown. |
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pHAMT35N/SK |
NPT-based plasmid, NPT driven by ham34 promoter, contains entire polylinker from pBluescript II SK+ |
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pTH209 |
Basic NPT plasmid, NPT driven by hsp70 promoter |
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pTH210 |
Basic HPT plasmid, HPT driven by hsp70 promoter. HPT can be released using SmaI and replaced with any desired sequence. |
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pHAMT35H |
Basic HPT plasmid, HPT driven by ham34 promoter. HPT can be released using SmaI and replaced with any desired sequence. |
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pHAMT35S |
Basic SPT plasmid, SPT driven by ham34 promoter. Note that some spontaneous streptomycin-resistant mutants may arise during transformation. |
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Library construction and general cloning (multiple cloning sites and/or other features) |
pTH209CL |
Cosmid vector derived from supercos-1, in which a NPT resistance gene driven by hsp70 promoter is inserted. |
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pHAMT35N/SK |
NPT-based plasmid, NPT driven by ham34 promoter, contains entire polylinker from pBluescript II SK+ |
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pTH209-BAC |
NPT-based plasmid for the contruction of bacterial artificial chromosome libraries, derived from BeloBac11 |
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Promoter analysis |
pNPGUS |
Promoter-less GUS plasmid for testing promoter fragments. Contains NPT gene for selection. Also known as Clone38.2 |
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Generalized expression or gene silencing |
pSTORA |
NPT-based gene silencing plasmid. Contains an additional promoter and terminator flanking the Ste20 intron from P. infestans, plus rare-cutting cloning sites, to facilitate the construction of hairpin genes |
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pTEP3 |
NPT-based plasmid. Uses Ham34 promoter to express additional sequences which can be cloned into a modest multiple cloning site (SmaI, SpeI, BamHI). |
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pTEP5 |
NPT-based plasmid. Uses Ham34 promoter to express additional sequences which can be cloned into a modest multiple cloning site (SacI, NotI, SpeI, EcoRI, SmaI). |
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Reporter expression |
pHAMT35G |
GUS driven by ham34 promoter. No selectable marker gene. |
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pTH209-35G |
5'Hsp70::NPT and 5'Ham34::GUS on the same plasmid |
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pTAF-G1 |
NPT-based plasmids for making fusions between GFP and a desired protein. The ham34 promoter can be used to express the protein of interest, or ham34 can be replaced by the native promoter. Both N and C-terminal fusions are possible. |
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pTAF-C1 |
CFP version of pTAF-G1 |
being finalized |
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pTAF-Y1 |
YFP version of pTAF-G1 |
being finalized |
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pTAF-mC |
mCherry version of pTAF-G1 |
being finalized |
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Protein pull-downs |
pXTAG |
NPT-based plasmid that expresses a desired protein with two tags (for TAP-tag purification schemes). Cloning region (ClaI-EcoRV-EcoRI-TAGS-SpeI-XbaI-NotI-SacII) allow N or C-terminal fusions to be made. Target protein is expressed from Ham34 promoter, but this can be replaced with the native promoter. Tag does not contain a nuclear localization signal. |
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