click map Judelson lab pages Transformation pages www.ucr.edu
The following plasmids have been used successfully in Phytophthora infestans. To request a plasmid or additional
information, please contact Howard Judelson (howard.judelson@ucr.edu). NOTE: We recently discovered a potential sequence rearrangement in some of our hpt-based plasmids. While this does not appear to affect the functionality of the plasmids, we are currently checking out their sequences. Please contact us if you have a question.

Category

Plasmid Name

Description

Map (pdf) and sequence (text file, abbreviated gbk format): will open in new window

Basic transformation (limited cloning sites)


Not all plasmids described in our publications are listed here. Please let us know if you are interested in a plasmid that is not shown.



pHAMT35N/SK

NPT-based plasmid, NPT driven by ham34 promoter, contains entire polylinker from pBluescript II SK+

map

sequence


pTH209

Basic NPT plasmid, NPT driven by hsp70 promoter

map

sequence


pTH210

Basic HPT plasmid, HPT driven by hsp70 promoter. HPT can be released using SmaI and replaced with any desired sequence.

map

sequence


pHAMT35H

Basic HPT plasmid, HPT driven by ham34 promoter. HPT can be released using SmaI and replaced with any desired sequence.

map

sequence


pHAMT35S

Basic SPT plasmid, SPT driven by ham34 promoter. Note that some spontaneous streptomycin-resistant mutants may arise during transformation.

map

sequence

Library construction and general cloning (multiple cloning sites and/or other features)

pTH209CL

Cosmid vector derived from supercos-1, in which a NPT resistance gene driven by hsp70 promoter is inserted.

map

sequence


pHAMT35N/SK

NPT-based plasmid, NPT driven by ham34 promoter, contains entire polylinker from pBluescript II SK+

map

sequence


pTH209-BAC

NPT-based plasmid for the contruction of bacterial artificial chromosome libraries, derived from BeloBac11

map

sequence

Promoter analysis

pNPGUS

Promoter-less GUS plasmid for testing promoter fragments. Contains NPT gene for selection. Also known as Clone38.2

map

sequence

Generalized expression or gene silencing

pSTORA

NPT-based gene silencing plasmid. Contains an additional promoter and terminator flanking the Ste20 intron from P. infestans, plus rare-cutting cloning sites, to facilitate the construction of hairpin genes

map

sequence


pTEP3

NPT-based plasmid. Uses Ham34 promoter to express additional sequences which can be cloned into a modest multiple cloning site (SmaI, SpeI, BamHI).

map

sequence


pTEP5

NPT-based plasmid. Uses Ham34 promoter to express additional sequences which can be cloned into a modest multiple cloning site (SacI, NotI, SpeI, EcoRI, SmaI).

map

sequence

Reporter expression

pHAMT35G

GUS driven by ham34 promoter. No selectable marker gene.

map

sequence


pTH209-35G

5'Hsp70::NPT and 5'Ham34::GUS on the same plasmid

map

sequence

Fluorescent protein fusions


pGFPN

For making N- or C-terminal fusions of proteins with GFP, using either the ham34 promoter or a promoter of your choice. Backbone confers G418 resistance (nptII)

map

sequence


pCFPN

For making N- or C-terminal fusions of proteins with CFP, using either the ham34 promoter or a promoter of your choice. Backbone confers G418 resistance (nptII)

map

sequence


pYFPN

For making N- or C-terminal fusions of proteins with YFP, using either the ham34 promoter or a promoter of your choice. Backbone confers G418 resistance (nptII)

map

sequence


pMCherryN

For making N- or C-terminal fusions of proteins with mCherry, using either the ham34 promoter or a promoter of your choice. Backbone confers G418 resistance (nptII)

map

sequence


pGFPH

For making N- or C-terminal fusions of proteins with GFP, using either the ham34 promoter or a promoter of your choice. Backbone confers hygromycin resistance (hpt)

map

sequence


pCFPH

For making N- or C-terminal fusions of proteins with CFP, using either the ham34 promoter or a promoter of your choice. Backbone confers hygromycin resistance (hpt)

map

sequence


pYFPH

For making N- or C-terminal fusions of proteins with YFP, using either the ham34 promoter or a promoter of your choice. Backbone confers hygromycin resistance (hpt)

map

sequence


pMCherryH

For making N- or C-terminal fusions of proteins with mCherry, using either the ham34 promoter or a promoter of your choice. Backbone confers hygromycin resistance (hpt)

map

sequence

Protein pull-downs

pXTAG

NPT-based plasmid that expresses a desired protein with two tags (for TAP-tag purification schemes). Cloning region (ClaI-EcoRV-EcoRI-TAGS-SpeI-XbaI-NotI-SacII) allow N or C-terminal fusions to be made. Target protein is expressed from Ham34 promoter, but this can be replaced with the native promoter. Tag does not contain a nuclear localization signal.

map

sequence