DNA PREPS
Alkaline lysis E. coli DNA preps
E. coli boiling prep miniscreen
P. infestans genomic DNA
Cleaning up genomic DNA using CTAB
Quantitating DNA with Biorad fluorometer
RNA PREPS
RNA extraction from P. infestans
CLONING AND MISC. DNA MANIPULATIONS
Primer and oligonucleotide design strategies
Restriction digestion of DNA
Cloning and sequencing PCR products
General advice on ligations and cloning
SSCP analysis
Chemically competent E. coli cells
Electroporation of E. coli
Making electrocompetent E. coli DH5-alpha (Eppendorf protocol)
Recycling electroporation cuvettes
Plating lambda libraries
Lambda plaque lifts
POLYMERASE CHAIN REACTION
Standard PCR (and colony PCR)
RAPD analysis
PCR amplification from phage plaques
MEDIA
Amsco autoclave instructions
General autoclaving guidelines
Chinese media (Xu's)
Lima bean media (ALBA)
Formaldehyde (RNA) gels
Rye and green pea media
HYBRIDIZATIONS AND GELS
General information on DNA gels and photo documentation
RNA gels (formaldehyde method)
Northern blots using glyoxal
cDNA probes from total RNA
Preparing glyoxal for RNA gels
Southern blotting of DNA (including making probes by random priming, and hybridization)
Hybridizations using an oligonucleotide as probe
PHYTOPHTHORA TRANSFORMATION
Phytophthora bombardment protocol
Phytophthora electroporation protocol (zoospores)
Protoplast transformation of P. infestans
MANIPULATIONS OF PHYTOPHTHORA
Determining mating types
Making zoospores and cysts
Harvesting sporulating and nonsporulating tissue
Making crosses and germinating oospores
Infecting leaflets with P. infestans
Freezing P. infestans in liquid nitrogen
REPORTER ASSAYS
GUS staining (histochemical)
GUS assays (fluorometric)
MISCELLANEOUS METHODS
Changing liquid nitrogen tanks
Recovering bacteria from microtiter plate stocks
G-force and rpm conversions for rotors in lab
Removing photographic fixer stains from clothing
Centrifuge rotors: use and cleaning
How to use the Rainin Pipetman
Rainin's Pipetman guide (includes replacement part numbers)
How to keep a good laboratory notebook
Advice on writing scientific papers
Using the moticam on the microscope
SAFETY
Safety training document for lab
Wipe test instructions
Departmental Chemical hygiene plan (2.1 Mb)
Departmental Injury and Illness Prevention Program
Flame and Alcohol Safety
MISCELLANEOUS FORMS
Radioactivity request and log sheet
Log sheet for working in radioactivity area
Log sheet for working in transfer hood