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Transformation protocols

Here are some of our current protocols related to transformation (P. infestans). Currently, our lab is using both the protoplast and electroporation (v. 2019) methods. Bombardment is rarely employed, but may be useful for species that are difficult to protoplast, do not make zoospores, or can not be cultured.

Microprojectile bombardment

Zoospore electroporation (original method, replaced by 2019 protocol)

Zoospore electroporation (revised 2019, use this one)

Protoplast transformation


Transformation workshop at UC-Riverside.

revised 8/2022