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General and contact information

Howard Judelson's background
education and interests

The oomycetes
learn more about these exciting organisms

The late blight disease
learn more about the problems that P. infestans causes

Research Interests
Ongoing research projects

Other lab members


Opportunities for graduate study in the lab

Promoter analysis

A major goal of the laboratory is to understand how Phytophthora regulates the expression of stage-specific genes, such as those transcribed only during sporulation or during zoospore formation.

We are also interested in understanding the overall function of the transcriptional machinery, including the architecture of intergenic regions and core promoters.

This work has combined bioinformatics and wet-lab experiments. Using bioinformatic tools, we mined the promoters of co-regulated genes for over-represented and evolutionarily conserved motifs. Fusions to reporter genes such as beta-glucuronidase (GUS) or green fluorescent protein (GFP) then confirmed the function of the motifs. Biochemical approaches are then used to purify the transcription factors that bind the motifs

Spatial patterns of expression of several spore-specific promoters, as revealed using transformants expressing the GUS marker gene

Demonstration that a 7-nt "cold box" is responsible for the induction of genes during cold-induced zoosporogenesis. This sequence was identified through the testing mutagenized versions of the PinfC promoter in transgenic P. infestans.

Spatial distribution of a novel oomycete core promoter sequence.

EMSA assay performed using wild type and mutant versions of binding regions from zoosporogenesis-specific promoter

Schema used to identify over-represented motifs and mark high-priority candidates for functional analysis.