General and contact information
Howard Judelson's background
education and interests
learn more about these exciting organisms
The late blight disease
learn more about the problems that P. infestans causes
Ongoing research projects
Other lab members
Opportunities for graduate study in the lab
A major goal of the laboratory is to understand how Phytophthora regulates the expression of stage-specific genes, such as those transcribed only during sporulation or during zoospore formation.
We are also interested in understanding the overall function of the transcriptional machinery, including the architecture of intergenic regions and core promoters.
This work has combined bioinformatics and wet-lab experiments. Using bioinformatic tools, we mined the promoters of co-regulated genes for over-represented and evolutionarily conserved motifs. Fusions to reporter genes such as beta-glucuronidase (GUS) or green fluorescent protein (GFP) then confirmed the function of the motifs. Biochemical approaches are then used to purify the transcription factors that bind the motifs
Spatial patterns of expression of several spore-specific promoters, as revealed using transformants expressing the GUS marker gene
Demonstration that a 7-nt "cold box" is responsible for the induction of genes during cold-induced zoosporogenesis. This sequence was identified through the testing mutagenized versions of the PinfC promoter in transgenic P. infestans.
Spatial distribution of a novel oomycete core promoter sequence.
EMSA assay performed using wild type and mutant versions of binding regions from zoosporogenesis-specific promoter
Schema used to identify over-represented motifs and mark high-priority candidates for functional analysis.